Figure 2.

CQ inhibits the functional activity of osteoclasts: (A) FITC-conjugated phalloidin and DAPI were used to stain F-Actin and the nuclei, respectively. At a 10X magnification, images were taken using a fluorescent microscope (Imager.Z2 Zeiss microscope). (B) Number of F-actin rings. (C) Number of nuclei per osteoclast. (D) Area of F-actin rings. ANOVA was used to analyse the results, and then unpaired Student t-tests were used to compare the indicated groups. Values are expressed as mean ± SEM, and equivalent outcomes were observed in two independent experiments. For the indicated groups, p 0.05 *** p ≤ 0.001) was used as the cutoff for statistical significance. In the bar graphs, red is indicating the positive control and green is indicating the treatment groups.