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. 2023 Jan 11;15(2):459. doi: 10.3390/cancers15020459

Figure 3.

Figure 3

CircZBTB46 promotes cell proliferation and cell cycle progression of AML. (A) qRT–PCR showing the significant knockdown of circZBTB46 in HL-60 cells via the transfection of siRNAs. (B) qRT–PCR showing the significant overexpression of circZBTB46 in K562 cells by the transfection of the circRNA-specific lentiviral plasmid. (C) CCK-8 assay showing the proliferation ability of HL-60 cells under control conditions (si-NC) or upon circZBTB46 knockdown (si-circZBTB46). (D) CCK-8 assay showing the proliferation ability of K562 cells after the transfection of the control vector or circZBTB46 overexpression vector. (E) The percentages of transfected HL-60 cells at different cell cycle phases (G1, S, and G2) were analyzed using flow cytometry. (F) The percentages of transfected K562 cells at different cell cycle phases (G1, S, and G2) were analyzed using flow cytometry. Data were presented as mean ± SD. Two-tailed p values were determined with either an unpaired Student’s t test (B,F) or a one-way (A,E) or two-way ANOVA (C,D); ** p < 0.01, *** p < 0.001, **** p < 0.0001. See also Figure S3.