Figure 1.

Experimental protocols. The first protocol (A) was performed to study differences of the LPS induced ALI between WT littermate and Esm1^−/− C57Bl/6 mice. The intra tracheal instillation of LPS was performed at D0, the clinical examination, with noninvasive measurement of oximetry, was performed each day, and the mice were sacrificed at different times to describe the features of ALI at each timing. The second protocol (B) was performed to study the effect of the continuous infusion of mouse endocan by osmotic pumps in Esm1^−/− mice. Osmotic pumps were filled with either endocan or PBS and implanted subcutaneously one day before i.t. LPS instillation. Clinical examination, including noninvasive measurement of oximetry, was performed daily, and the mice were sacrificed at day 5 to describe the features of ALI. LPS: lipopolysaccharides; i.t.: intra tracheal instillation.