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. 2023 Jan 12;15(2):489. doi: 10.3390/cancers15020489

Figure 6.

Figure 6

B activates ROS/JNK mediated apoptosis and autophagy in Caco2 cells. (a) The relative percentage cell viability of Caco2 cells detected after treatment with B (20 µg/mL) or EL000327 (60 µg/mL) for 48 h, with or without NAC (5 mM) or the JNK inhibitor SP600125 (10 µM). (b) Flow cytometric analysis of dead cells stained by Annexin V-FITC and PI after treatment with B (20 µg/mL) or EL000327 (60 µg/mL) for 48 h, with or without NAC (5 mM) or SP600125 (10 µM). (c) Quantification of the percentage of apoptotic cells after treatment with the indicated concentrations of B or EL000327 for 48 h with or without NAC and SP600125 and analyzed by flow cytometry. (d) Western blot analysis of apoptosis, autophagy, and the JNK signaling pathway related protein expression in Caco2 cells treated with B (20 µg/mL) or EL000327 (60 µg/mL) for 24 or 48 h, with or without SP600125 (10 µM). (e) Quantification of Bcl-XL and p62 protein expressions in the presence or absence of SP600125. Data represent the mean ± S.D. *** p < 0.001 compared with the NAC- and SP600125-treated groups or the DMSO-treated control. NS: no significant difference.