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. 2023 Jan 4;12(2):221. doi: 10.3390/cells12020221

Figure 1.

Figure 1

Rapamycin produces a long-lasting inhibition of mTOR activity. (A) Representative pictures showing the immunofluorescence of the protein PS6RP, which is a downstream enzymatic product of mTOR. Rapamycin produces a marked and long-lasting decrease of the PS6RP immunofluorescence. Densitometry of the PS6RP immunofluorescence (B) and the representative PS6RP immunoblotting (C) indicate that rapamycin induces a massive mTOR inhibition, which lasts at least 14 days following rapamycin withdrawal. Data in (B) are given as the mean percentage ± SEM of optical density measured in 100 cells per group (assuming controls as 100%). * p < 0.05 compared with controls. Scale bar = 12 μm.