Figure 1.

Schematic diagram showing (a) Hydrodynamic injection into the tail vein of C57BL/6J/129Sv mice. Briefly, 2 mL volume of empty vector or saline solution (control) was hy-drodynamically injected into the tail vein of anaesthesized C57BL/6J/129Sv mice. (b) Liver tissue was resected at different time points and quickly fixed with buffered 1% osmium tetroxide. For electron microscopic evaluation, ultrathin sections were cut from glycidether embedded blocks.