Figure 5.

The effects of CBP and PL MSCs exosomes on the surface markers profile of the expanded CD34+. A total of 2 × 10⁴ CB CD34⁺ cells were cultured (pre-expansion) and maintained under three different culture conditions for 10 days (n = 3, 3 replicates each). Flow cytometric analysis for surface markers CD34, CD38, CD45, and Lin (surface markers cocktail of differentiated cells) was done for day 10 expanded cells: (a) Representative diagram for immunophenotype of subpopulations of expanded CB CD34⁺ cells; (b) representative diagram for immunophenotype of subpopulations of expanded CB CD34⁺ CD38⁻ cells; (c) representative diagram for immunophenotype of subpopulations of expanded CB CD34⁺ CD38⁻ Lin⁻ cells; (d) representative diagram for immunophenotype of subpopulations of expanded CB CD45⁺ Lin⁺ cells. * p < 0.001 compared with the control group. The fold increase represents the increase over the pre-expansion cells that were cultured.