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Western blotting to confirm reactivity to the RpoS component of the GST fusion product. (A) Western blot probed with anti-C. burnetii RpoS serum and reacted with undigested GST-RpoS (lane 1) or thrombin-digested GST-RpoS (lane 2); (B) Western blot probed with anti-RpoS antibody and reacted with undigested GST-RpoS (lane 1) or GST-RpoS digested with thrombin for 2 h (lane 2) or overnight (lane 3).
