Figure 2.

Effects of dilution of primary amplification product on secondary PCR. The amplified target was gadR. The primary PCR was performed as described in Table 2. Lane 1: primary PCR product; lanes 2–6: secondary PCR products using 0, 10, 100, 1000, and 10,000-fold diluted primary products as templates, respectively; lane 7: secondary PCR result without primary product; and lane M: DNA 5000 Marker (5000, 3000, 2000, 1500, 1000, 750, 500, 250, and 100 bp).