Figure 2.

Analysis of epirubicin fluorescence emission via conventional flow cytometry (BD FACSVerse). MDA-MB-231 cells treated for 24 and 48 h with 1 µM (A) or 2.5 µM (B) epirubicin were acquired using flow cytometry and shown as blue histograms on every channel of a conventional instrument (FACSVerse, BD Biosciences), equipped with three lasers (488 nm, 633 nm, and 405 nm). Overlayed red histograms show the related untreated samples. Histograms are representative of three independent experiments.