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. 2022 Dec 28;45(1):164–174. doi: 10.3390/cimb45010013

Figure 3.

Figure 3

Analysis of doxorubicin fluorescence emission with ImageStream. MDA-MB-231 cells treated for 24 h with 1 µM doxorubicin were acquired using ImageStream: (A) representative images of treated cell brightfield and fluorescence detected in channel 1 (FITC and analogue fluorochromes) and channel 4 (propidium iodide and analogue fluorochromes) for representative cells treated with doxorubicin (Doxo) or untreated are shown; (B) histograms represent doxorubicin autofluorescence detected in channel 1 (FITC and analogue fluorochromes) and channel 4 (propidium iodide and analogue fluorochromes). Data are representative of three independent experiments.