Fig 1. Effect of terahertz radiation on activity of the primary hippocampal neuron.

Primary rat hippocampal neurons were isolated and cultured as described in materials and methods. (A), primary hippocampal cells showing neuronal staining with MAP2 (green). Nuclei were stained with DAPI (blue) (scale bar = 50 μm). Cellular activity was measured by CCK-8 assay immediately or 1 h after indicated terahertz radiation. The cellular activities were shown in B and C after exposure to 0.12 THz with power of 10 mW for 10 min and 30 min, respectively. Moreover, cellular activities were also presented in D and E after radiated by 0.157 THz with power of 50 mW for 10 min and 30 min respectively. Sham-radiated cells under the same conditions were used as the controls. Three independent experiments were conducted. Data were shown as mean±s.e.m. *p<0.05, **p<0.01 vs corresponding group.