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. 2023 Jan 20;18(1):e0267064. doi: 10.1371/journal.pone.0267064

Fig 2. Effect of terahertz radiation on the apoptosis and ultrastruture of primary hippocampal neuron.

Fig 2

Rat hippocampal neurons were radiated by 0.12 THz with power of 10 mW for 30 min or by 0.157 THz with power of 50 mW for 30 min. Apoptosis was analyzed by flow cytometry using Annexin V and propidium iodide staining immediately after terahertz radiation. The representative images were shown in A, and the statistical analysis were shown in B. Sham-irradiated cells were used as the controls. Three independent experiments were conducted. Data were shown as mean±s.e.m. **p<0.01 vs sham group. The ultrastructure of the cell was examined by transmission electron microscopy (TEM) after exposure to sham-radiation (C), 0.12 THz radiation with power of 10 mW for 30 min (D), or 0.157 THz radiation with power of 50 mW for 30 min (E). Organelles are indicated by arrows, green: nucleus; red: mitochondria; blue: ER; yellow: lysosome. Scale bars are 500 nm in C, and 2 μm in D and E.