Table 1.

AS-PCR Primers for PIK3CA E542K and E545K mutation detection.

Mutation	Reverse Primers/Template Fragment	Abbreviation
E542K Nucleotide (c.1624G>A) Amino Acid (p.E542K) COSMIC ID (COSV55873227)	5′-AAATCTTTCTCCTGCTCAGTGATGTT-3′	TGTT
	WT DNA 5′-AAATCTTTCTCCTGCTCAGTGATTTC-3′	-
	Mutant DNA 5′-AAATCTTTCTCCTGCTCAGTGATTTT-3′	-
E545K Nucleotide (c.1633G>A) Amino Acid (p.E545K) COSMIC ID (COSV55873239)	5′-GACTCCATAGAAAATCTTTCTCCTGGTT-3′	GGTT
	WT DNA 5′-GACTCCATAGAAAATCTTTCTCCTGCTC-3′	-
	Mutant DNA 5′-GACTCCATAGAAAATCTTTCTCCTGCTT-3′	-

Boldly marked nucleotides represent mismatched nucleotides in relation to the WT DNA sequence.