Table 4.
PIK3CA mutations detection by AS-PCR using various mutation percentages (total 104 copies per reaction).
| Primers | Cq | ΔCq = CqWT − Cq% | ||||
|---|---|---|---|---|---|---|
| WT | 0.5% | 1% | WT–0.5% | WT–1% | ||
| E542K | TGTT | 33.9 ± 0.5 | 33.2 ± 0.3 | 32.8 ± 0.2 | 0.7 | 1.1 |
| T*GTT | 37.3 ± 0.5 | 36.2 ± 0.2 | 35.4 ± 0.3 | 1.1 | 1.9 | |
| TC*TT | 39.5 ± 0.6 | 37.4 ± 0.1 | 36.9 ± 0.1 | 2.1 | 2.6 | |
| T*CTT | 38.0 ± 0.7 | 36.2 ± 0.4 | 36.0 ± 0.2 | 1.8 | 2.0 | |
| T*TTT | 35.7 ± 0.3 | 34.7 ± 0.2 | 34.7 ± 0.4 | 1.0 | 1.0 | |
| E545K | GGTT | 30.9 ± 0.1 | 30.7 ± 0.1 | 30.6 ± 0.1 | 0.2 | 0.3 |
| GG*TT | 41.4 ± 0.6 | 39.5 ± 0.3 | 38.4 ± 0.1 | 1.9 | 3.0 | |
| G*GTT | 38.9 ± 0.6 | 36.5 ± 0.1 | 35.9 ± 0.1 | 2.4 | 3.0 | |
| GA*TT | 37.6 ± 0.7 | 36.2 ± 0.1 | 35.8 ± 0.2 | 1.4 | 1.8 | |
| G*ATT | 41.2 ± 0.6 | 39.4 ± 0.4 | 38.7 ± 0.2 | 1.8 | 2.5 | |
| G*CTT | 42.5 ± 0.7 | 39.5 ± 0.1 | 38.5 ± 0.1 | 3.0 | 4.0 | |
No template control (NTC) was undetermined in all the reactions. The symbol “*” indicates the PG modification location. Boldly-marked nucleotides represent mismatched nucleotides concerning the WT DNA sequence.