Table 5.
Validation of AS primers on DNA samples from FFPE.
| Primer/Mutation | ΔCq Cut-Off | Sensitivity 1 | Specificity 2 |
|---|---|---|---|
| GGTT E545K | 2.84 | 56%/83% 3 | 100% |
| GG*TT E545K | 10.55 | 67%/100% | 100% |
| G*GTT E545K | 8.00 | 78%/100% | 100% |
| G*CTT E545K | 10.64 | 67%/100% | 100% |
| TGTT E542K | 4.31 | 86%/100% | 100% |
| TC*TT E542K | 7.67 | 86%/100% | 100% |
| T*CTT E542K | 7.84 | 86%/100% | 100% |
1 Sensitivity value = true-positive samples/(false-negative + true-positive samples) × 100%; 2 Specificity value = true-negative samples/(false-positive + true-negative samples) × 100%; 3 Sensitivity recalculation excluding low VAF and low DNA amount samples (lower than 300 DNA copies per µL). For calculations, see Tables S3 and S4.