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. 2023 Jan 19;15(1):2163838. doi: 10.1080/19490976.2022.2163838

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Caspase-8 and NLRP3 inhibitors reduced NLRP3 inflammasome, apoptosis and pyroptosis in AIEC colonized IL10^−/− mice after piroxicam feeding. (a) RT-qPCR of colonic NLRP3 inflammasome, and caspases. (b) IL-1β protein expression. (c) Western blot of Caspase-8, inflammasome and cell death markers. (d) Western blot of N4BP1. n = 3–7/group. Data are presented as mean ± SEM. Significance was determined using one-way ANOVA with Bonferroni or Kruskal–Wallis test with Dunn’s multiple comparison test, respectively. * p < .05; ** p < .01; *** p < .001.