Table 1. Antibodies and inhibitors.
WB, Western blot; IP, immunoprecipitation; IF, immunofluorescence; PLA, proximity ligation assay.
| Antibodies | |||
|---|---|---|---|
| Antigen | Species | Catalog no./source | Application |
| FLAG | Mouse | F3165; Millipore Sigma | WB, IP |
| HPV16 L1 | Mouse | MAB885; Millipore Sigma | WB |
| α-COP | Rabbit | HPA028024; Millipore Sigma | WB |
| β-COP | Rabbit | A304-724A; Thermo Fisher Scientific | WB, IP |
| β′-COP | Rabbit | PA5-96557; Invitrogen | WB, IP, IF |
| γ-COP | Rabbit | 12393-1-AP; Proteintech | WB, IP, IF, PLA |
| δ-COP | Rabbit | 23843-1-AP; Proteintech | WB |
| Arf1 | Rabbit | 10790-1-AP; Proteintech | WB |
| TGN46 | Rabbit | ab50595; Abcam | WB, PLA |
| GM130 | Rabbit | ab52649; Abcam | WB, IF, PLA |
| EEA1 | Rabbit | C45B10; Cell Signaling | WB |
| BAP31 | Rat | MA3-002; Invitrogen | WB |
| Na+/K+-ATPase | Rabbit | ab76020; Abcam | WB |
| Histone H3 | Rabbit | 9715S; Cell Signaling | WB |
| GFP | Mouse | 632380; Takara Bio Clontech | WB |
| SGTA | Rabbit | 11019-2-AP; Proteintech | WB |
| β-Actin | Rabbit | 4967S; Cell Signaling | WB |
| Hsp90 | Mouse | sc13119; Santa Cruz Biotechnology | WB |
| HA | Rat | ROAHAHA; Roche | WB |
| HPV16 L1 | Mouse | 554171; BD | PLA |
| SV40 T antigen | Mouse | sc-147; Santa Cruz Biotechnology | IF |
| VPS35 | Rabbit | Ab157220; Abcam | WB |
| Other antibodies | Species | Catalog no./source | Application |
| Anti-mouse IgG peroxidase | Goat | A4416; Millipore Sigma | WB |
| Anti-rabbit IgG peroxidase | Goat | A4914; Millipore Sigma | WB |
| Anti-rat IgG peroxidase | Rabbit | A5795; Millipore Sigma | WB |
| Anti-rabbit Alexa Fluor 488 | Goat | A11008; Thermo Fisher Scientific | IF |
| Anti-mouse Alexa Fluor 594 | Goat | A11032; Thermo Fisher Scientific | IF |
| Normal rabbit IgG | Rabbit | NI01; Millipore Sigma | IP |
| Inhibitors | |||
| Compound | Solvent | Catalog no./source | Concentration |
| XXI | DMSO | 565790; Millipore Sigma | 1–2 μM |
| NAV | DMSO | SML2238; Millipore Sigma | 10–25 μM |