Table 1.
Hormone | Measurement Method | Kit & Instrument | Intra-Assay | Inter-Assay |
---|---|---|---|---|
Total testosterone (TT) | enzyme immunoassay (EIA) | Diagnostic biochem Canada Co., London, ON, Canada | 1.7% | 2.3% |
Dehydroepiandrosterone sulfate (DHEAS) | enzyme immunoassay (EIA) | DRG Instruments, GmbH, Marburg, Germany | 1.9% | 2.5% |
Sex hormone-binding globulin (SHBG) | immunoenzymometric assay (IEMA) | Diagnostic biochem Canada Co., London, ON, Canada | 0.8% | 2.4% |
Luteinizing hormone (LH) | immunoradiometric assay (IRMA) | commercial kits (Izotop, Budapest, Hungary) and a Dream Gamma- 10 gamma counter (Shin Jin Medics Inc., Koyang, Korea) | 1.6% | 4.2% |
follicle-stimulating hormone (FSH) | 1.4% | 2% | ||
thyroid-stimulating hormone (TSH) * | 2.1% | 3.1% | ||
FT4 * | electrochemiluminescence immunoassay (ECLIA) | commercial kits on the Cobas e411 analyzer (Roche Diagnostics GmbH, Mannheim, Germany) | 1.3% | 3.3% |
* The sensitivity of FT4 and TSH measurements were 0.023 ng/dl and 0.005 mU/L, respectively.