Table 2.
List of primers and their sequences for molecular markers (ISSR and SCoT) and plastid rbcL and matK genes.
| Primer Code | Sequence (5′-3′) | Size (bp) |
|---|---|---|
| ISSR marker | ||
| ISSR-807 | (AG)8 T | 172–1156 |
| ISSR-810 | (GA)8 T | 159–2949 |
| ISSR-835 | (Ag)8 YC | 96–4039 |
| ISSR-841 | (GA)8 YC | 102–420 |
| ISSR-857 | (AC)8 YG | 84–244 |
| ISSR-825 | (AC)7 T | 327–2562 |
| ISSR-814 | (CT)7 CAT | 215–496 |
| ISSR-826 | (AC)8 C | 223–1808 |
| ISSR-827 | (AC)8 G | 255–1408 |
| ISSR-840 | (gA)8 TT | 123–1164 |
| SCoT marker | ||
| SCoT 1 | CAACAATGGCTACCACCC | 513–3099 |
| SCoT 2 | ACCATGGCTACCACCGGC | 296–3658 |
| SCoT 3 | CAACAATGGCTACCACGC | 183–2079 |
| SCoT 4 | CAACAATGGCTACCACCG | 177–3009 |
| SCoT 5 | ACGACATGGCGACCACGC | 142–669 |
| SCoT 6 | CCATGGCTACCACCGCAG | 331–2274 |
| Plastid rbcL and matK genes | ||
| rbcL | F: 5′-ATGTCACCACAAACAGAGACTAAAGC-3′ | 600 |
| R: 5′-TCGCATGTACCTGCAGTAGC-3′ | ||
| matK | F: 5′-CGATCTATTCATTCAATATTTC-3′ | 900 |
| R: 5′-TCTAGCACACGAAAGTCGAAGT-3′ | ||
F: Forward primer and R: Reverse primer.