Table 6.
Number of amplified bands, number of polymorphic bands, polymorphism percentage, mean of band frequency and marker efficiency calculations of the 16 ISSR and SCoT markers assayed for the 16 T. aestivum genotypes studied.
Molecular Marker | MB | Polymorphic Bands | TAB | PPB | MBF | H | PIC | E | MI | H.av | D | Rp | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UB | NUB | NPB | ||||||||||||
ISSR-807 | 1 | 7 | 0 | 7 | 8 | 88 | 0.5 | 0.50 | 0.37 | 3.8 | 0.02 | 0.00 | 0.78 | 2.9 |
ISSR-810 | 2 | 6 | 0 | 6 | 8 | 75 | 0.6 | 0.49 | 0.37 | 4.5 | 0.02 | 0.00 | 0.69 | 3.3 |
ISSR-835 | 4 | 5 | 2 | 7 | 11 | 64 | 0.7 | 0.44 | 0.34 | 7.4 | 0.02 | 0.00 | 0.55 | 3.6 |
ISSR-841 | 3 | 3 | 1 | 4 | 7 | 57 | 0.5 | 0.49 | 0.37 | 3.9 | 0.02 | 0.00 | 0.70 | 1.7 |
ISSR-857 | 0 | 9 | 1 | 10 | 10 | 100 | 0.3 | 0.44 | 0.35 | 3.3 | 0.01 | 0.00 | 0.89 | 4.9 |
ISSR-825 | 1 | 5 | 0 | 5 | 6 | 83 | 0.5 | 0.50 | 0.37 | 3.1 | 0.02 | 0.01 | 0.73 | 2.7 |
ISSR-814 | 0 | 2 | 1 | 3 | 3 | 100 | 0.3 | 0.39 | 0.31 | 0.8 | 0.01 | 0.01 | 0.93 | 1.2 |
ISSR-826 | 3 | 7 | 0 | 7 | 10 | 70 | 0.8 | 0.35 | 0.29 | 7.7 | 0.02 | 0.00 | 0.40 | 2.4 |
ISSR-827 | 1 | 5 | 2 | 7 | 8 | 88 | 0.5 | 0.50 | 0.37 | 3.7 | 0.02 | 0.00 | 0.78 | 2.3 |
ISSR-840 | 0 | 7 | 1 | 8 | 8 | 100 | 0.3 | 0.39 | 0.31 | 2.1 | 0.01 | 0.00 | 0.93 | 4.1 |
Total | 15 | 56 | 8 | 64 | 79 | - | - | 4.50 | 3.47 | 40.4 | 0.15 | 0.04 | 7.38 | 29.2 |
Mean | 1.5 | 5.6 | 0.8 | 6.4 | 7.9 | 82.41 | 0.49 | 0.45 | 0.35 | 4.04 | 0.01 | 0.00 | 0.74 | 2.92 |
SCoT 1 | 0 | 8 | 0 | 8 | 8 | 100 | 0.3 | 0.40 | 0.30 | 2.6 | 0.01 | 0.00 | 0.9 | 3.3 |
SCoT 2 | 2 | 9 | 0 | 9 | 11 | 82 | 0.6 | 0.50 | 0.40 | 6.3 | 0.02 | 0.00 | 0.7 | 5.1 |
SCoT 3 | 1 | 9 | 1 | 10 | 11 | 91 | 0.3 | 0.50 | 0.40 | 3.9 | 0.01 | 0.00 | 0.9 | 5.7 |
SCoT 4 | 0 | 11 | 2 | 13 | 13 | 100 | 0.3 | 0.40 | 0.30 | 3.5 | 0.01 | 0.00 | 0.9 | 6.5 |
SCoT 5 | 0 | 10 | 0 | 10 | 10 | 100 | 0.4 | 0.50 | 0.40 | 4.5 | 0.01 | 0.00 | 0.8 | 7.1 |
SCoT 6 | 0 | 7 | 4 | 11 | 11 | 100 | 0.2 | 0.40 | 0.30 | 2.8 | 0.01 | 0.00 | 0.9 | 4.9 |
Total | 3 | 54 | 7 | 61 | 64 | - | - | 2.70 | 2.10 | 23.6 | 0.07 | 0.02 | 5.1 | 32.7 |
Mean | 0.5 | 9 | 1.17 | 10.17 | 10.70 | 95.46 | 0.36 | 0.40 | 0.30 | 3.9 | 0.01 | 0.00 | 0.9 | 5.4 |
Total of All | 18 | 110 | 15 | 125 | 143 | - | - | 7.2 | 5.5 | 64.0 | 0.21 | 0.06 | 12.5 | 61.9 |
Mean of All | 1.2 | 7.33 | 1 | 8.33 | 9.53 | 88.94 | 0.43 | 0.4 | 0.3 | 4.0 | 0.01 | 0.00 | 0.8 | 3.9 |
MB, Monomorphic bands; UB, unique bands; NUB, non-unique bands; NPB, number of polymorphic bands; TAB, total amplified bands; PPB, percentage of polymorphic bands; MBF, mean of band frequency; H, heterozygosity index; E, effective multiplex ratio; PIC, polymorphism information content; H.av, average expected heterozygosity; D, discriminating power; MI, marker index; Rp, resolving power.