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. 2022 Dec 22;14(1):34. doi: 10.3390/genes14010034

Table 6.

Number of amplified bands, number of polymorphic bands, polymorphism percentage, mean of band frequency and marker efficiency calculations of the 16 ISSR and SCoT markers assayed for the 16 T. aestivum genotypes studied.

Molecular Marker MB Polymorphic Bands TAB PPB MBF H PIC E MI H.av D Rp
UB NUB NPB
ISSR-807 1 7 0 7 8 88 0.5 0.50 0.37 3.8 0.02 0.00 0.78 2.9
ISSR-810 2 6 0 6 8 75 0.6 0.49 0.37 4.5 0.02 0.00 0.69 3.3
ISSR-835 4 5 2 7 11 64 0.7 0.44 0.34 7.4 0.02 0.00 0.55 3.6
ISSR-841 3 3 1 4 7 57 0.5 0.49 0.37 3.9 0.02 0.00 0.70 1.7
ISSR-857 0 9 1 10 10 100 0.3 0.44 0.35 3.3 0.01 0.00 0.89 4.9
ISSR-825 1 5 0 5 6 83 0.5 0.50 0.37 3.1 0.02 0.01 0.73 2.7
ISSR-814 0 2 1 3 3 100 0.3 0.39 0.31 0.8 0.01 0.01 0.93 1.2
ISSR-826 3 7 0 7 10 70 0.8 0.35 0.29 7.7 0.02 0.00 0.40 2.4
ISSR-827 1 5 2 7 8 88 0.5 0.50 0.37 3.7 0.02 0.00 0.78 2.3
ISSR-840 0 7 1 8 8 100 0.3 0.39 0.31 2.1 0.01 0.00 0.93 4.1
Total 15 56 8 64 79 - - 4.50 3.47 40.4 0.15 0.04 7.38 29.2
Mean 1.5 5.6 0.8 6.4 7.9 82.41 0.49 0.45 0.35 4.04 0.01 0.00 0.74 2.92
SCoT 1 0 8 0 8 8 100 0.3 0.40 0.30 2.6 0.01 0.00 0.9 3.3
SCoT 2 2 9 0 9 11 82 0.6 0.50 0.40 6.3 0.02 0.00 0.7 5.1
SCoT 3 1 9 1 10 11 91 0.3 0.50 0.40 3.9 0.01 0.00 0.9 5.7
SCoT 4 0 11 2 13 13 100 0.3 0.40 0.30 3.5 0.01 0.00 0.9 6.5
SCoT 5 0 10 0 10 10 100 0.4 0.50 0.40 4.5 0.01 0.00 0.8 7.1
SCoT 6 0 7 4 11 11 100 0.2 0.40 0.30 2.8 0.01 0.00 0.9 4.9
Total 3 54 7 61 64 - - 2.70 2.10 23.6 0.07 0.02 5.1 32.7
Mean 0.5 9 1.17 10.17 10.70 95.46 0.36 0.40 0.30 3.9 0.01 0.00 0.9 5.4
Total of All 18 110 15 125 143 - - 7.2 5.5 64.0 0.21 0.06 12.5 61.9
Mean of All 1.2 7.33 1 8.33 9.53 88.94 0.43 0.4 0.3 4.0 0.01 0.00 0.8 3.9

MB, Monomorphic bands; UB, unique bands; NUB, non-unique bands; NPB, number of polymorphic bands; TAB, total amplified bands; PPB, percentage of polymorphic bands; MBF, mean of band frequency; H, heterozygosity index; E, effective multiplex ratio; PIC, polymorphism information content; H.av, average expected heterozygosity; D, discriminating power; MI, marker index; Rp, resolving power.