Table 1.
Sequences of primers and PCR conditions used for genotyping of PKCγ variants rs923331350 and rs1331232028.
| Variant rs IDs | Primer Sequences | Denaturation | Annealing | Extension |
|---|---|---|---|---|
| rs923331350 G/T |
Forward inner primer (G allele): GTTTTGCAGAAAGGAGG | 95 °C | 51 °C | 72 °C |
| Reverse inner primer (T allele): ACCTTCTGCCTCAGAGA | ||||
| Forward outer primer (5′-3′): CTCGGAATTTCCCTGT | ||||
| Reverse outer primer (5′-3′): AGTCGGGACTACAGCC | ||||
| rs1331232028 G/A |
Forward inner primer (G allele): TTCCTCATGGTTCTAGGCAG |
95 °C | 57 °C | 72 °C |
| Reverse inner primer (A allele): ACCTTCCCAAAACTGCATT | ||||
| Forward outer primer (5′-3′): GGTAGGAGGGTGGCCA | ||||
| Reverse outer primer (5′-3′): CCGTCCCCTCAAGGAG |