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. 2022 Dec 20;14(1):5. doi: 10.3390/genes14010005

Figure 1.

Figure 1

CR reduced β-cell area and insulin production in regular diet-fed mice. (a) Body weight curves of calorie-restricted NCD-fed mice. (b) Weight of the pancreas. (c) Intraperitoneal glucose tolerance test. (d) Insulin levels were measured at 15 min after glucose injection. (e) HE staining of pancreatic sections. (f) Quantification of islet size. (g) Insulin mRNA. (h) Immunofluorescence staining for insulin (insulin, green) in the pancreases of NCD or a diet restricted to 70% of the calories of the ad libitum group (NCR). Nuclei were stained with DAPI (blue). (i) Quantification of insulin. The results were expressed as mean values ± SEM. (Five mice per group) * p < 0.05 vs. NCD mice.