Figure 3.

CR increased the lipogenic gene expression in pancreas of normal chow diet-fed mice. (a) Representative immunofluorescence images of fatty acid synthase (FASN, red) and insulin (insulin, green). (b) Quantification of FASN-positive β-cells/total β-cells. (c) Representative Western blot of mouse pancreas. PPARγ, SREBP1c, ACC, and FASN were measured. Quantification of PPARγ, SREBP1c, ACC, and FASN. (d) PPARγ, SREBP1c, ACC, and FASN mRNA levels. (e) Pancreatic triglyceride. The results were expressed as mean values ± SEM. (n = 5 per group) * p < 0.05 vs. NCD mice.