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. 2022 Dec 23;14(1):45. doi: 10.3390/genes14010045

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cell growth, γ-H2AX foci formation, and nuclear accumulation of Nrf2 staining (A) The number of cells at 3 days after irradiation in indicated samples. Asterisk displays a significant difference in IR+ melatonin group and IR+ MitoEbselen-2 group compared with that of IR groups at same Gy level. (B) γ-H2AX foci (red) and nuclear accumulation of Nrf2 (green) were shown in irradiated TIG-3 cells. Scale bar = 50 µm. Fluorescence intensity of γ-H2AX (C) and Nrf2 (D) is shown in the graph. Asterisk displays a significant change in fluorescence intensity values of the staining compared with that of non-irradiated control cells.