Table 2.
Primers for porcine MSMB.
| Primer Name | Primer Sequence (5′-3′) | Amplified Fragment Size (bp) | Annealing Temperature (°C) | Application |
|---|---|---|---|---|
| MSMB-1 | F: GATGCACATGCCTGAAAGGACTC | 668 | 55 | Exon 1 |
| R: GAGTGATGGTTCCCAATTTGCTGA | ||||
| MSMB-2 | F: GTCCAACAGATCCATATCAGCCTA | 858 | 59 | Exon 2 |
| R: CTGTCCCAACCCTTTCTCTCATATA | ||||
| MSMB-3 | F: ATCACCCTAAATGCCCCTGACTCA | 778 | 57 | Exon 3 |
| R: TGAAAGAGGCATAGCTGTCCTTAG | ||||
| MSMB-4 | F: CCTACCTGGAGTGACTGACACATA | 676 | 61 | Exon 4 |
| R: AGTTAGAGGCCTAGGGAATGAGG | ||||
| MSMB | F: AAAGAAGGACCCAGGAAAG | 145 | 60 | Real-time PCR |
| R: CAATCATAGACAGTTAGAGGC | ||||
| GAPDH | F: TTGTGATGGGCGTGAACC | 169 | 58 | Reference gene |
| R: GTCTTCTGGGTGGCAGTGAT |
Note: F stands for upstream primer and R stands for downstream primer.