FIG. 4.

Colony lift immunoblot of isolate 6050 colonies before and after recovery from an infected-tissue chamber. Isolates were recovered from chamber fluid aspirates obtained on day 7 or 14 postinfection, diluted, and plated on solid media to obtain individual colonies. Colonies from the original and recovered isolates were overlaid with nitrocellulose membranes, which were probed for SpeA expression using anti-SpeA antibodies as described in Materials and Methods. The blots shown are representative of a minimum of five different experiments with blots performed on bacteria recovered on different days.