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. 2023 Jan 8;14(1):172. doi: 10.3390/genes14010172

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Subcellular localization of PHF8-PS. (A) Schematic representation of nuclear localization signals (NLS) and nuclear export signals (NES) in PHF8 and PHF8-PS proteins (image created with Biorender.com). (B) Cell fractionation of NIH3T3 cells transiently expressing HA/FLAG-tagged PHF8 and PHF8-PS proteins. Whole cell lysate (W), nuclear (N), and cytoplasmic (C) fractions were analyzed by Western blot as indicated. (C) Co-immunostaining images of NIH3T3 cells transiently expressing HA/FLAG-tagged PHF8 and PHF8-PS showing the subcellular localization of these proteins (green) and that of the mitochondrial marker Tom20 (red). Cell nuclei were stained DAPI (blue). Scale bar: 10 μm.