FIG. 3.

SEB-induced IFN-γ and IL-8 production in whole blood. Whole blood was stimulated with SEB (2 μg/ml) in the absence or in the presence of IL-18BP (125 ng/ml), TNFbp (10 μg/ml), or anti-IL-12 Ab (2.5 μg/ml) (aIL-12). After 24 h the culture was assayed for IL-8 (B), and after 48 h it was assayed for IFN-γ (A). The amount of either IL-8 or IFN-γ induced by SEB was set at 100% for each donor and the percent change (mean ± SEM) for each condition is shown. A 100% SEB stimulation represents 143 ± 55 ng/ml (IFN-γ) and 66 ± 12 ng/ml (IL-8). ∗, P < 0.05 versus SEB alone (n = 6 donors).