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. 2023 Jan 20;20:13. doi: 10.1186/s12985-023-01961-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — A Plot the one-step growth curve of the virus. B Infection by the isolated viruses passaged up to passage 12 was observed microscopically for 60 h. C ORF3 recombinant plasmids were prepared to generate a standard curve. D Viral load assays were performed at 60 h, 72 and 84 h of CH/HLJJS/2022 infection (n = 3, P > 0.005)