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. 2022 Dec 19;25(1):68–78. doi: 10.1038/s41556-022-01037-0

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — (a) Tip1:GFP intensity on the microtubule lattice as well as on the microtubule tip depends non-linearly on the concentration of Tea2. (b) The intensity of Mal3-Alexa647 is less effected by the Tea2 concentration. Each data point in panels a and b was obtained by averaging tip and lattice intensities at the corresponding concentration, as shown in c and d, respectively. The number of observed intensity profiles obtained from one experiment per condition is indicated in the legend of panel c (the same number for Tip1:GFP and Mal3-647 and for ‘tip’ and ‘lattice’, but different at different Tea2 concentrations as indicated). Data are presented as mean ± S.D. (c,d) Intensity profiles of Tip1:GFP (c) and Mal3-647 (d) in the Tea2 titration experiments. Intensity profiles were extracted from TIRF microscopy images of dynamic microtubules.

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