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. 2022 Dec 19;25(1):68–78. doi: 10.1038/s41556-022-01037-0

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Examples of individual Mal3, Tea2 and Tip1 intensity profiles at two different concentrations of Tea2 (20 nM and 100 nM). b, Averaged Tea2-Alexa647 intensity profiles. c, Averaged Tip1:GFP intensity profiles. d, Averaged Mal3-Alexa647 intensity profiles. Data in c and d were extracted from the same experiment for each Tea2 concentration, whereas Tea2-Alexa647 data shown in b were recorded in two separate experiments that contained dark Tip1 and Mal3-Alexa488. Profiles were averaged for microtubules of similar length. See also Extended Data Fig. 4. e, Ratio of lattice (left) and comet (right) intensities between 100 nM and 20 nM Tea2 for microtubules of all lengths. The total number of observed intensity profiles at 100 nM is indicated for each condition. Numerical data are available in source data.

Source data