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. 2022 Dec 19;25(1):68–78. doi: 10.1038/s41556-022-01037-0

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© The Author(s) 2022, corrected publication 2023

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Fig. 5 — a, Condensates formed by full-length Mal3 and Mal3 truncations (1 µM) in the presence of 5% PEG-35k. b, Unlabelled FLMal3 (200 nM, scaffold) tagged with FL-Mal3:mCherry (2 nM, tag) was allowed to recruit Mal3:GFP (2 nM, client) in the presence of PEG-6k. Graphs show distributions of tag and scaffold Mal3 intensities (arrow denotes the threshold applied for speckle detection in images). c, Intensity of Mal3:GFP (full-length or truncated constructs) recruited to FL-Mal3 scaffold droplets. Lines show median, and numbers in the shaded area (N) show number of droplets analysed (one experiment per construct). d,e, Kymographs showing end-tracking by Mal3/Tip1/Tea2 in the presence of full-length Mal3 (d) or Mal3-ΔIDR2 (e) and in the absence of PEG. f,g, Intensities of Mal3-Alexa647 (f) and Tip1:GFP (g) in the comets forming in the presence of FL-Mal3 or Mal3-ΔIDR2. Lines show median, and numbers in the shaded area (N) show number of comets analysed (pooled across two independent experiments per condition). Numerical data are available in source data.

Source data