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. 2022 Dec 19;25(1):56–67. doi: 10.1038/s41556-022-01035-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a-c, Close-up views of the α (a), β (b), and γ (c) interfaces seen between protomers in the NcKar9N crystal (PDB ID 7AG9). Interacting residue side chains are shown in stick representation. Oxygen and nitrogen atoms are colored in red and blue, respectively; carbon atoms are in dark green for chain A and in light green for chains B, C, and D. Interface residues that were mutated in the three crystallographic dimers are labeled in bold and blue lettering. d and e, Far-ultraviolet CD spectra (d) and thermal denaturation profiles (e) of His-NcKar9N (red), His-NcKar9N-α⁻ (blue), His-NcKar9N-β⁻ (yellow), and His-NcKar9N-γ⁻ (magenta). Inset in (e), SDS-PAGE analysis of the His-NcKar9N variants. Spectra are averages of 3 measurements of the same sample, denaturation profiles are single experiments. Related to Fig. 2.