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. 2022 Dec 19;25(1):56–67. doi: 10.1038/s41556-022-01035-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Trajectories of microtubule (MT) length (top) and Kar9-mNeonGreen fluorescence intensity (bottom) extracted during individual microtubule growth (left) and shrinkage (right) phases in wild-type cells. b, Estimates of initial microtubule length (top; defined as the length at the start of a growth or shrinkage phase) and growth/shrinkage rate (bottom) during microtubule growth (left) and shrinkage (right) phases using linear regression for individual trajectories in the indicated strains. Dots, estimates for individual trajectories; long lines, medians; short lines, 50% quantiles. c, As panel b but for fluorescence intensity of Kar9 puncta. d, Durations of microtubule growth and shrinkage phases. P values were determined by Wilcoxon rank sum test. For panels b-d, blue values indicate n = number of microtubule growth/shrinkage events analyzed, single experiment. e, As Fig. 5f,g, but showing the linear mixed-effects (LME) model estimates of initial microtubule lengths and Kar9-mNeonGreen fluorescence intensity levels at the start of microtubule growth and shrinkage phases. Color of P values (two-sided t-test) and n (number of timeframes considered for the model, single experiment) matches the growth/shrinkage category they refer to. Related to Fig. 5. Source numerical data available in source data.

Source data