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. 2022 Dec 19;25(1):56–67. doi: 10.1038/s41556-022-01035-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a, Micrographs of mNeonGreen-Bim1 and mNeonGreen-Bik1 expressing control and 5% hexanediol treated cells as quantified in Fig. 7e. Since these cells did not express CFP-Tub1, the transmission channel is used as a reference in magenta. Scale bar, 3 μm. b, Micrographs of GFP-Tub1 expressing control, 5% hexanediol, and 30 μg/mL nocodazole treated cells. c, Frequency of cells with visible astral microtubules labeled with GFP-Tub1 in control, 5% hexanediol, and 30 μg/mL nocodazole treatment (error bars are Wilson/Brown 95% confidence intervals determined from binomial distribution). P values above bars are determined by two-proportion z test. d, Micrographs of cells expressing Kar9-mNeonGreen treated with 30 μg/mL nocodazole versus control. e, Kar9-mNeonGreen fluorescence intensity levels in wild-type cells treated with 5% hexanediol or 30 μg/mL nocodazole versus untreated control (normalized median with 95% confidence intervals, the control and 5% hexanediol data are the same as shown in Fig. 7e). P values were determined by Welch’s t-test. Blue values in panels c,e indicate n = number of cells analyzed, single experiment. Related to Fig. 7d,e. Source numerical data available in source data.

Source data