Fig. 3. Impact of mutations in core Kar9-network components on phase separation in vitro.
a, Superimposed phase diagrams of different Kar9-interface mutants with Bim1 in the presence (light blue) or absence (dark blue) of Bik1. For comparison, the phase diagrams of Kar9 + Bim1 + Bik1 (dashed light-blue line) and Kar9 + Bim1 (dashed dark-blue line) equimolar mixtures (Fig. 1d) are also shown in all plots. Single experiment. b,c, Phase contrast micrographs illustrating phase behaviour for the indicated protein mixtures and mutants at 220 mM NaCl and 10 μM protein. Scale bar, 10 μm. Source numerical data available in source data.