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. 2022 Dec 19;25(1):56–67. doi: 10.1038/s41556-022-01035-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — Time series (left) displaying fluorescence recovery in the indicated protein droplets after a bleaching laser pulse and quantification (right). Tenfold less concentrated labeled protein (mNeonGreen-Bik1, Bim1-GFP, or Kar9-GFP, indicated with green letter fonts) was added to mixtures of unlabeled protein (5 μM each). Blue line displays fluorescence intensity over time of a single experiment normalized to prebleached droplet intensity, grey dotted line is a single exponential decay fitted to the recovery period. Half-lives (t_1/2) of the fitted decay are given with 95% confidence intervals of the fit. For Bik1 droplets, the quantified intensity was corrected for the effect of defocusing according to unbleached reference droplets. Scale bar 3 μm. Single experiment. Related to Fig. 1. Source numerical data available in source data.

Source data