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. 2022 Dec 19;25(1):56–67. doi: 10.1038/s41556-022-01035-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Partition coefficients (ratio between fluorescence intensity inside and outside of droplets) for mNeonGreen-Bik1, Bim1-GFP, Kar9-GFP, and mNeonGreen into non-fluorescent, equimolar mixtures of Kar9 + Bim1 + Bik1, Kar9 + Bim1, and Bik1 droplets (10 μM each; median with 95% confidence interval). Blue values indicate n analyzed droplets, single experiment. b, Phase diagram of Kar9 that was titrated into an equimolar mixture of Bim1 + Bik1 (10 μM each). Single experiment. Related to Fig. 1. Source numerical data available in source data.

Source data