Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 9;25(1):108–119. doi: 10.1038/s41556-022-01042-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 7 — (a) Resolved soluble (S) and insoluble (P) fractions from WM983B cells subject to the indicated extractions were examined by western blotting with antisera raised against LAP1, GAPDH or Lamin B1. Western blot representative of N = 2 independent experiments. (b) Schematic of the different N-terminal domains of LAP1 isoforms and cartoon illustrating the mitochondrial retargeting assay. (c) Representative images of mitochondrial retargeting assay in cells expressing mEmerald-Lamin A/C (left) or mEmerald-Lamin B1 (right) and stained for HA (magenta) and DNA (blue). Scale bars, 10 μm. (d) Percentage of cells showing co-localisation of mEmerald-Lamin A/C (left, n = 210, 211 and 190 cells, respectively) or mEmerald-Lamin B1 (right, n = 211, 199 and 183 cells, respectively) with LAP1B^NT/ LAP1B^1–121/LAP1C^NT. Bar charts show the mean and error bars represent S.E.M. from N = 3 independent experiments. P-values calculated by one-way ANOVA (d); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Unprocessed western blots and numerical data are available in the Source Data.

Source data