Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 9;25(1):108–119. doi: 10.1038/s41556-022-01042-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a, Percentage of primary melanoma WM983A and metastatic melanoma WM983B cells grown in 2D displaying NE blebs. b, Representative images of WM983B nuclei with NE blebs of different shapes stained for Lamin A/C (magenta) and DNA (blue). Scale bars, 10 μm. c, Percentage of nuclei with NE blebs according to bleb number per nucleus and bleb shape in WM983A and WM983B cells. n = 434 and 561 cells, respectively. d, Percentage of NE blebs containing Lamin A/C, Lamin B1 or Lamin B2 in WM983A and WM983B cells. n = 605 and 510 cells, respectively. e, Representative images of a WM983B nucleus with an NE bleb stained for Lamin A/C (green), Lamin B1 (magenta) and DNA (blue). Scale bar, 10 μm. f, Representative live-cell image sequence of WM983B GFP-NLS nuclei with an intact NE bleb (left) and a ruptured NE bleb (right) with correlative serial block-face scanning electron microscopy (SBF-SEM) images of the same nuclei. The white arrow indicates the site of rupture of the nuclear membranes. Scale bar, 5 μm. g, Percentage of intact and ruptured NE blebs in WM983A and WM983B cells imaged over the course of 15 h. n = 486 and 453 cells, respectively. h, Percentage of intact and ruptured NE blebs in WM983A (n = 486 and 522 cells, respectively) and WM983B (n = 453 and 560 cells, respectively) cells stably expressing GFP-NLS after treatment with GSK269962A and imaged over the course of 15 h. i, Duration of NE rupture in WM983A and WM983B cells stably expressing GFP-NLS and imaged live (not significant (NS), P = 0.8389). j, NE rupture events per hour in WM983A and WM983B stably expressing GFP-NLS and imaged live. n = 486 and 453 cells, respectively. In a, c, d, g and h, bar charts show the mean and error bars represent s.e.m. from N = 3 independent experiments. In i and j, horizontal lines show the median and whiskers show minimum and maximum range of values. P values calculated by two-way ANOVA (c, d, g and h) and two-tailed unpaired t-test (a, i and j); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Numerical data and exact P values are available in source data.

Source data