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. 2023 Jan 9;25(1):108–119. doi: 10.1038/s41556-022-01042-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a,b, Representative images and QuPath mark-up (map) of haematoxylin and eosin (HE) staining (a) and quantification of invading cells into the dermis (b) from day 42 WM983A and WM983B tumours grown in NXG mice. Dashed lines represent the boundary between PIF and DIF. HE-positive tumour cells are in red in the map. n = 16 and 16 tumours, respectively. c,d, Representative images and QuPath mark-up of HE staining (c) and quantification of invading cells into the dermis (d) from day 24 and day 36 A375P tumours and day 24 A375M2 tumours grown in NXG mice (d). n = 8, 8 and 16 tumours, respectively. e,f, Representative images (e) and percentage of tumour cell nuclei according to LAP1 intensity score in TB, PIF and DIF (f) of day 42 WM983A and WM983B tumours. n = 16 and 15 tumours, respectively. g,h, Representative images (g) and percentage of tumour cell nuclei according to LAP1 intensity score in TB, PIF and DIF (h) of day 24 and day 36 A375P tumours and day 24 A375M2 tumours. n = 8, 8 and 16 tumours, respectively. i–k Representative and QuPath mark-up of HE staining (i), quantification of invading cells into the dermis (j) and tumour growth curves (k) of A375P GFP-NLS LAP1B-mRuby3, A375P GFP-NLS LAP1B^Δ1-72-mRuby3, A375P GFP-NLS LAP1B^R563G-mRuby3, A375P GFP-NLS LAP1C-mRuby3 and A375M2 GFP-NLS tumours grown intradermally in NXG mice. n = 10 tumours per condition. In IHC panels, scale bars are 50 μm. In b, d and j, horizontal lines show the median and whiskers show minimum and maximum range of values. In k, graphs show the mean and error bars represent s.d. P values calculated by one-way ANOVA (d and j), two-way ANOVA (f and h) and two-tailed unpaired t-test (b). In f and h, significant P values shown are for LAP1 score 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Numerical data and exact P values are available in source data.

Source data