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. 2023 Jan 9;25(1):108–119. doi: 10.1038/s41556-022-01042-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, Representative images of TB and IF of a primary tumour and a metastasis in human melanoma patient cohort A. Scale bars, 50 μm. The magnifications show representative cell nuclei. Scale bars, 10 μm. b, H-score for LAP1 staining in TB and IF of primary tumours and metastases in cohort A by unpaired (left) or paired (right) analysis. c, Percentage of tumour cell nuclei according to LAP1 intensity score in TB and IF of primary tumours and metastases in patient cohort A. Significant P values shown are for LAP1 score 3. n = 19 primary tumours and 14 metastases. d, Representative images of TB and IF of a primary tumour and a metastasis in human melanoma patient cohort B. Scale bars, 50 μm. The magnifications show representative cell nuclei. Scale bars, 10 μm. e, H-score for LAP1 staining in TB and IF of primary tumours and metastases in patient cohort B by unpaired (left) or paired (right) analysis. f, Percentage of tumour cell nuclei according to LAP1 intensity score in TB and IF of primary tumours and metastases in patient cohort B. Significant P values shown are for LAP1 score 3. n = 29 primary tumours and 29 metastases. g, Kaplan–Meier survival curve of disease-free survival according to LAP1 expression in the IF from primary melanomas. LAP1 expression was categorized as low or high using the mean expression. n = 46 primary melanomas. h, Summary model. LAP1 levels are elevated at the IF of melanoma tumours, which are enriched in cells displaying amoeboid features like a rounded cell morphology, high levels of myosin II and NE blebs. LAP1C, but not LAP1B, can localize to NE blebs in a Lamin-A/C-dependent manner and promotes transit through physical constraints through its weaker N-terminal NE/lamina tethering allowing NE blebbing. In b and e, horizontal lines show the median and whiskers show minimum and maximum range of values. P values calculated by one-way ANOVA (b and e), two-way ANOVA (c and f), two-tailed unpaired and paired t-test (b and e) and log-rank test (g); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Numerical data and exact P values are available in source data.

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