Extended Data Fig. 2. Extended Data Fig. 2, related to Fig. 2, RT-DC and OS.
(A-D) Real-time deformability scatter plots (deformation versus cell size in μm2) of PTRFKO MEFs reconstituted with PTRF [A], Cav1 [B] or PTRFKO MEFs without reconstitution [C]. Color indicates a linear density scale; a 50%-density contour plot of the three genotypes is shown [D]. Measurements were recorded at a flow rate of 0.32 μl/s (M3) in a 20 μm × 20 μm channel. (E) Optical Stretcher measurement scheme (strain in % vs time in seconds), showing the deformation (laser on) and relaxation regions. (F and G) Compliance and strain across different genotypes after 10 s stretching, right before relaxation. Statistical comparisons were by one-way ANOVA with Bonferroni post-test, with significance assigned at *p < 0.05 and not significant at p > 0.05. The number of total cells analyzed per genotype (N) is indicated, pooled from 4 independent experiments. Source numerical data are available in source data.