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. 2023 Jan 21;13:17. doi: 10.1038/s41398-023-02307-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Venn diagram showing the total number of expressed genes in each group in the large circles, and the number of genes expressed in both groups are shown in the overlapping portion of the circles. B Volcano map showing the significantly differentially expressed genes represented by red dots (upregulated) and green dots (downregulated) for the VPA_TD_FMT group and the VPA_ASD_FMT group. C KEGG enrichment analysis showing the top 20 signaling pathways affected by DEGs between the VPA_TD_FMT group and the VPA_ASD_FMT group. D Reactome annotation analysis of the DEGs between the VPA_TD_FMT group and the VPA_ASD_FMT group. Twenty statistically significant pathways are listed. E Correlation between the changes in the relative abundances of individual genera and DEGs involved in the serotonergic synapse pathway and glutamatergic synapse pathway in the colon tissue of the VPA_TD_FMT group and the VPA_ASD_FMT group. P-values were corrected for multiple testing using the Benjamini‒Hochberg false discovery rate. *p < 0.05, **p < 0.01.