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. 2023 Jan 20;14:341. doi: 10.1038/s41467-023-35922-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative confocal images of HeLa EXD2^+/+, EXD2^−/− and EXD2^−/− + EXD2^WT-cl1 mock- or UV-irradiated (15 J/m²) and left to recover for an hour. Cells were labeled with anti-EXD2 and anti-FLAG and stained with MitoTracker. Images of the cells were obtained with the same microscopy system and constant acquisition parameters for a given labeling/staining. b EXD2^+/+ cells were mock- or UV-irradiated (15 J/m²) and let to recover for 1 h. Cells were fractionated in mitochondria (Mito) and chromatin (Chro) fractions, which were resolved by SDS-PAGE and immunoblotted against the indicated proteins. Molecular sizes are indicated (KDa). ATP5A is a marker of mitochondria. Histone H3 is a marker of chromatin. Source data are provided as a Source Data file. c EXD2^−/−-cl1 or EXD2^−/− + EXD2^WT-cl1 cells were mock- or UV-irradiated (15 J/m²) and let to recover for the indicated period of time post-UV (RT). RNAPII was immunoprecipitated using anti-RPB1 from total extracts and protein were resolved by SDS-PAGE and immunoblotted using anti-RPB1 or anti-EXD2 antibodies. HC antibody heavy chain, LC antibody light chain, RT recovery time. Molecular sizes are indicated (KDa). Source data are provided as a Source Data file. d. RNAPII was immunoprecipitated from chromatin fractions obtained in panel b, using anti-RPB1 in the presence of Benzonase. IP using IgG was performed as controls. Proteins were resolved by SDS-PAGE and immunoblotted using anti-RPB1 or anti-EXD2 antibodies. RT recovery time. Molecular sizes are indicated (KDa). Source data are provided as a Source Data file. e Purified RNAPII from HeLa cells³⁹ was incubated with recombinant pulldown GST-EXD2^WT. Following washes, fractions were resolved by SDS-PAGE and immunoblotted against the indicated proteins. Controls IP was performed with GST alone (lane 4). Molecular sizes are indicated (KDa). Source data are provided as a Source Data file.