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. 2023 Jan 20;13:1147. doi: 10.1038/s41598-023-28395-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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BPA promoted lipid accumulation in LO2 cells. (a) Different concentrations of BPA (10, 100, 1000, 10,000 nM) were added to LO2 cells for 24 h, and MTT assay was performed to assess the cell viability. (b) BODIPY 493/503 staining was performed for lipid droplets. Scale bar = 20 μm. (c) The relative quantification of BODIPY 493/503 fluorescence intensity. (d) TG levels and (e) cholesterol levels in LO2 cells. The mRNA expression levels of (f) PPARγ, (g) SREBF1, (h) FASN and (i) DGAT-1 were measured by qRT-PCR. Data were expressed as the mean ± SD of 3 independent experiments. *p < 0.05 and **p < 0.01, compared with control group.