Table 1.
Over-representation analysis of 1,2-DCVC exposure across cell models for low and high concentrations
| Low concentrations | |||
|---|---|---|---|
| q-value | % genes overlap* | Pathway | Pathway source |
| 1.96E-07 | 10.4 | NRF2 pathway | Wikipathways |
| 2.25E-05 | 4.8 | Nuclear Receptors Meta-Pathway | Wikipathways |
| 5.89E-05 | 18.2 | Porphyrin and chlorophyll metabolism—(human) | KEGG |
| 0.000425441 | 23.1 | Glucuronidation | Reactome |
| 0.000472089 | 21.4 | Codeine and Morphine Metabolism | Wikipathways |
| 0.000576877 | 18.8 | Pentose and glucuronate interconversions—(human) | KEGG |
| 0.000658341 | 8.4 | Phase II—Conjugation of compounds | Reactome |
| 0.000658341 | 16.7 | Ascorbate and aldarate metabolism—(human) | KEGG |
| 0.000658341 | 15.8 | Ferroptosis—(human) | KEGG |
| 0.004668623 | 7.5 | Drug metabolism—other enzymes—(human) | KEGG |
| High concentrations | |||
|---|---|---|---|
| q-value | % genes overlap* | Pathway | Pathway source |
| 3.90E-05 | 39 | NRF2 pathway | Wikipathways |
| 0.00019879 | 66.7 | Photodynamic therapy-induced unfolded protein response | Wikipathways |
| 0.000285382 | 81.8 | Response of EIF2AK1 (HRI) to heme deficiency | Reactome |
| 0.000285382 | 25.4 | Cellular responses to stress | Reactome |
| 0.000500637 | 39.6 | Protein processing in endoplasmic reticulum—(human) | KEGG |
| 0.001395995 | 57.9 | Ferroptosis—Homo sapiens (human) | KEGG |
| 0.002145114 | 37 | IL-17 signaling pathway—(human) | KEGG |
| 0.004475598 | 100 | Cysteine Metabolism | SMPDB |
| 0.005007318 | 36 | p53 signaling pathway—(human) | KEGG |
| 0.015686101 | 44 | Validated transcriptional targets of TAp63 isoforms | PID |
Examples of 10 significant (q value ≤ 0.01) pathways from over-representation analysis among cell models after chemical exposure to 1,2-DCVC for 24 h. Upregulated DEGs across cell models upon 1,2-DCVC exposure were used as input for ORA statistical analysis. Low concentrations were considered the lowest concentration tested that resulted in at least one DEG for each cell model. High concentrations were considered as the top concentration tested in each cell model. *% genes overlap— % of DEGs in tested condition that overlap with pathway gene set list that are contained in the background list of genes (EUToxRisk v2.2)