Table 4:
Parameter values for secreted factors derived from literature and optimized using parameter estimation.
| Parameter | Description | Value | Unit | Measurement techniques | Citation |
|---|---|---|---|---|---|
| IL-1β parameters | |||||
| kIL-1β,CM | Secretion rate by cardiomyocytes | 7.55e-5 | pg/ml/cell/hour | ELISA | (Horton et al., 2006) |
| kIL-1β,N | Secretion rate by neutrophils | 0.06 | pg/ml/cell/hour | MILLIPLEX MAP mouse cytokine/chemokine panel | (McLoed et al., 2016) |
| kIL-1β,Mo | Secretion rate by monocytes | 9.58e-3 | pg/ml/cell/hour | Immunoblotting | (Gaidt et al., 2016) |
| kIL-1β,Mϕ | Secretion rate by macrophages | 0.015 | pg/ml/cell/hour | ELISA | (Meng & Lowell, 1997) |
| kIL-1β,F | Secretion rate by fibroblasts | 2.25e-3 | pg/ml/cell/hour | -- | Estimated value |
| kIL-1β,deg | Degradation rate | 0.4086 | 1/hour | Ribonuclease protection assay and curve fitting | (Dewald et al., 2004) |
| TNFα parameters | |||||
| kTNFα,CM | Secretion rate by cardiomyocytes | 5e-7 | pg/ml/cell/hour | ELISA | (Horton et al., 2006) |
| kTNFα,N | Secretion rate by neutrophils | 1.2e-3 | pg/ml/cell/hour | Immunofluorescence | (Finsterbusch et al., 2014) |
| kTNFα,Mo | Secretion rate by monocytes | 1.58e-4 | pg/ml/cell/hour | ELISA | (Matic & Simon, 1991) |
| kTNFα,Mϕ | Secretion rate by macrophages | 3.15e-4 | pg/ml/cell/hour | ELISA | (Minshawi et al., 2019) |
| kTNFα,F | Secretion rate by fibroblasts | 9.5e-5 | pg/ml/cell/hour | ELISA | (Del Re et al., 2010) |
| kTNFα,deg | Degradation rate | 0.4786 | 1/hour | Ribonuclease protection assay and curve fitting | (Deten & Zimmer, 2002) |
| GM-CSF parameters | |||||
| kGMCSF,N | Secretion rate by neutrophils | 9.6e-3 | pg/ml/cell/hour | Luminex assay | (Satoh et al., 2006) |
| kGMCSF,Mo | Secretion rate by monocytes | 1.95e-3 | pg/ml/cell/hour | Luminex assay | (Satoh et al., 2006) |
| kGMCSF,F | Secretion rate by fibroblasts | 5.24e-4 | pg/ml/cell/hour | Bio-plex assay | (Rossini et al., 2008) |
| kGMCSF,deg | Degradation rate | 3.15 | 1/hour | qRT-PCR and curve fitting | (Vandervelde et al., 2007) |
| MMP-9 parameters | |||||
| kMMP9,N | Secretion rate by neutrophils | 7.35e-4 | pg/ml/cell/hour | Gelatin zymography | (Chakrabarti et al., 2006) |
| kMMP9,Mo | Secretion rate by monocytes | 6.5e-5 | pg/ml/cell/hour | ELISA | (Nold et al., 2003) |
| kMMP9,Mϕ | Secretion rate by macrophages | 3e-5 | pg/ml/cell/hour | ELISA | (Bellosta et al., 2001) |
| kMMP9,F | Secretion rate by fibroblasts | 6.5e-5 | pg/ml/cell/hour | Immunoblotting | (Somanna et al., 2016) |
| kMMP9,deg | Degradation rate | 0.292 | 1/hour | Gelatin zymography | (F. Wang et al., 2007) |
| Latent TGFβ parameters | |||||
| klTGFB,CM | Secretion rate by cardiomyocytes | 5e-7 | pg/ml/cell/hour | ELISA | (Schindler et al., 1998) |
| klTGFB,Mϕ | Secretion rate by macrophages | 3.15e-4 | pg/ml/cell/hour | ELISA | (Schindler et al., 1998) |
| klTGFB,F | Secretion rate by fibroblasts | 9.5e-5 | pg/ml/cell/hour | Mink lung epithelial cell-bioassay | (Fisher & Absher, 1995) |
| klTGFB,deg | Degradation rate | 0.4786 | 1/hour | Ribonuclease protection assay and curve fitting | (Ikeuchi et al., 2004) |
| TGFβ parameters | |||||
| kTGFβ,act | Activation rate | 5e-7 | 1/hour | Estimated value from TGFβ activation function | (Jin et al., 2011) |
| kTGFβ,deg | Degradation rate | 0.4786 | 1/hour | Ribonuclease protection assay and curve fitting | (Ikeuchi et al., 2004) |
| Collagen and debris parameters | |||||
| kdebris | Debris conversion rate | 1 | 1/hour | -- | Estimated value |
| kproColl,F | Procollagen secretion rate by fibroblasts | 3.15e-4 | pg/ml/cell/hour | Hydroxyproline assay | (Mummidi et al., 2016) |
| kcollMat | Collagen maturation rate | 9.5e-5 | 1/hour | Immunoblotting | (Del Re et al., 2010) |
| kdeg,Coll | Degradation rate of collagen by MMP-9 | 0.4786 | 1/hour | -- | (Rikard et al., 2019) |