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. 2023 Jan 20;14:338. doi: 10.1038/s41467-023-36029-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Crossing scheme for determination of sds3G1-Cas9-induced inheritance bias. b, c Boxplots of kmo^sgRNAs inheritance (b) and eye phenotype (c) rates of I₂ larvae. The median value is indicated by a thick black line and the maximum and minimum ends of whiskers represent the most extreme data point that is no more than 1.5 times the interquartile range. The size of each symbol is scaled to the amount of I₂ progeny scored. Total number of screened larvae from each cross is presented below corresponding data points. Mosquito figures obtained from Ramirez^38,39. Source data are provided as a Source data file. pro = promoter, ♂ = male, ♀ = female.